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The formation of primer dimers is a particular problem when several PCR reactions are carried out in the same tube (multiplex PCR). Primer dimer formatin can occur even if there are several mismatches in the primer-dimer duplexes, which are stabilized by binding to Taq polymerase. Primer dimers are most likely to form at the beginning of PCR amplification, when the primers are present in high concentrations relative to the template. Incorrect amplicons are sometimes generated in PCR owing to primer dimer formation, in which the PCR primers hybridize, and are amplified instead of the template DNA ( Figure 4). Invented in the 1980s by Kary Mullis, the polymerase chain reaction has had such a ubiquitous effect on molecular biology, DNA diagnostics and forensic science that Mullis was awarded the Nobel Prize. In theory, n cycles of PCR will produce 2 n PCR products. In all subsequent cycles amplification produces PCR products of a length specified by the loci of the two primers, and these PCR products soon outnumber the original target molecules. The polymerase can only be extend the DNA as far as the locus of the first primer, producing DNA duplexes of a specific length. The second cycle of PCR is a repeat of the first cycle, and each newly synthesized single strand also acts as a template for primer annealing and extension. The PCR cycle A single cycle of the polymerase chain reaction This Taq polymerase is heat-resistant, meaning that temperatures of up to 95 ☌ can be used in PCR, conditions of low DNA duplex stability. While all organisms contain DNA polymerases, the polymerase that is used in PCR comes from the thermophilic bacterium Thermus aquaticus. The region of the template bound by the primers is amplified in a series of cycles ( Figure 1). 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Previous conference: American | New conference: Big 12 Charlotte will embark on its AAC adventure under a new coach after the 49ers hired Biff Poggi to replace Will Healy late last year. The two North Carolina programs have never clashed the gridiron. The 49ers now have some in-state competition, too, with East Carolina checking in among the AAC holdovers. Granted, the 49ers' new home will include plenty of familiar faces since they are one of six Conference USA schools making the jump to the AAC. Previous conference: Conference USA | New conference: AmericanĬonference USA is the only FBS league that Charlotte - a program revitalized in 2013 - has been part of since earning the promotion from the FCS in 2015. The Cougars' most recent conference home was the Mountain West (1998-2010). A healthy portion of BYU's history can be traced back to the Western Athletic Conference (1962-98) and the defunct Skyline Conference (1938-61). 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